Application of Multi-Dimensional Gas Chromatography Mass Spectrometry Techniques to Explain the Increased Sensitivity of Atlantic Haddock Embryos to Crude Oil Pollution

Lisbet Srensen1,2, Steven Rowland3, William Robson3, Michael Wilde3, Andy Booth4, Svein Mjs2, Sonnich Meier1


1

Environmental Chemistry Group, Institute of Marine Research, Bergen, Norway
2 Department of Chemistry, University of Bergen, Norway
3 Petroleum and Environmental Geochemistry Group, Biogeochemistry Research Centre, University of Plymouth, UK
4 SINTEF Materials and Chemistry, Trondheim, Norway

Presented at: 40th International Symposium on Capillary Chromatography and 13th GCGC Symposium, Riva del Garda, Italy, 29 May-3 June 2016.

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Introduction

Atlantic haddock embryos (Melanogrammus aeglefinus) are observed to be highly sensitive to contamination with dispersed crude oil compared to eggs of biologically similar species (e.g. Atlantic cod, Gadus morhua) [1]. Deleterious effects appear to correlate with the accumulation of oil droplets on the chorion of the haddock eggs, but causality has not been shown. In the current study, we investigated the uptake of both target compounds (PAHs) and unknown petroleum compounds in cod and haddock after 9 days of exposure to crude oil droplets (11-600 g/L seawater).

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