Microbial Toxicity and Degradation of a Monoaromatic Crude Oil Fraction

M. Frenzel1,2, S.J. Rowland2, A. Scarlett3, A.M. Booth2, T. Galloway3, S.K. Burton1 and H.M. Lappin-Scott1


School of Biosciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS, U.K.
2 Petroleum & Environmental Geochemistry Group, SEOES, University of Plymouth, Plymouth PL4 8AA, U.K.
3 Ecotoxicology & Stress Biology Research Group, University of Plymouth, Plymouth PL4 8AA, U.K.

Presented at: 160th Meeting of the Society for General Microbiology, Manchester, U.K., 26-29 March 2007.

Download: Poster as an A4 pdf file.

Brief Summary

Fractions of oil which are resistant to weathering represent large volumes of environmentally toxic waste comprise complex mixtures of chemicals. Such fractions are unresolved by conventional analytical methods. A bacterial consortium enriched from Whitley Bay sediment, UK has been previously reported to degrade alkylcyclohexyltetralins proposed as model compounds for such oil fractions. In the present study, a monoaromatic hydrocarbon fraction isolated from Venezuelan TJP crude oil was subjected to biodegradation by the Whitley Bay consortium. Hydrocarbon degradation, and changes in the UCM composition were quantified using GC-MS and GCxGC-TOF-MS. Microbial community stability was assessed by PCR-DGGE of the 16S rRNA gene. Microtox studies of the isolated fraction were performed before and after exposure to the Whitley Bay consortium using a Vibrio strain. This characterised microbial consortium is thus able to aerobically degrade these hitherto recalcitrant toxic oil fractions.

Download the complete poster as an A4 pdf file.

Top of Page Recent PEGG Posters PEGG Home Page